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Home > People > Faculty

Division of Water Environment

Zhou Xiaohong
Associate Professor
Tel: (86)-10-62796953
Location: School of Environment, Tsinghua University,Beijing,100084

Education Background

Ph.D. in Environmental engineering, July 2007, Tsinghua University, Beijing, China

B.S. in Environmental engineering, July 2002, Tongji University, Shanghai, China

Experience

2009-present Assistant Professor, Department of Environmental Science and Engineering, Tsinghua University 

2007-2009 Postdoctoral Associate, Department of Environmental Science and Engineering, Tsinghua University 

Areas of Research Interests/ Research Projects

Research Area:

1. Structure and function of biofilm

2. Microsensor design and application

3. Electrochemical sensor in environmental monitoring

Research Projects:

1. The effect of benzene and its substituted compounds on the nitrogen cycling in sediments, supported by China Postdoctoral Science Foundation, July, 2007-July, 2009.

2. Study on in-situ determination of biofilm kinetic parameters in water bodies, supported by China Postdoctoral Science Foundation, July, 2007-July, 2009.

3. Development of novel phosphate sensor for sensitive detection of phosphate in water samples, supported by Natural Science Foundation of Zhejiang province, Jan., 2009-Dec., 2010.

Academic Achievements

Jin, YS, Sun, ZJ, Gu, AZ, Zhou, XH. Proteomics technologies in toxicity screening: a review[J]. ENVIRONMENTAL CHEMISTRY LETTERS, Published 2025, Volume 23, Issue 1, Pages 67-80

Jiao, SP, Jin, YS, Lichtfouse, E, Zhou, XH. Hydrovoltaic technologies for self-powered sensing and pollutant removal in water and wastewater: a review[J]. ENVIRONMENTAL CHEMISTRY LETTERS, Published 2025

Qian, XN, Zhu, QY, Sun, WJ, Zhou, XH, Xiong, XY, Zeng, SY, Sheng, JW, He, M. A reagent-free on-site COD monitoring by a variable optical path UV-Vis spectrometer[J]. FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING Published 2025, Volume 19, Issue 5

Wang, YD, Chen, J, Gong, LK, Tang, JJ, Wang, XW, Guo, HY, Zhou, XH. P-doped W2C nanoparticles for hydrogen evolution reaction powered by a wind-driven triboelectric nanogenerator[J]. NANO ENERGY, Published 2024, Volume 121

Yang, YH, Sun, LL, Zhao, JH, Jiao, Y, Han, TL, Zhou, XH. Improving trans-cleavage activity of CRISPR-Cas13a using engineered crRNA with a uridinylate-rich 5′-overhang[J]. BIOSENSORS & BIOELECTRONICS, Published 2024, Volume 255

Wang, Bohan,Liu, Lanhua,Zhang, Haopeng,Wang, Zhiqiang,Chen, Kang,Wu, Bo,Hu, Limin,Zhou, Xiaohong,Liu, Lanlan. A group-targeting biosensor for sensitive and rapid detection of quinolones in water samples[J]. Analytica Chimica Acta, Published 2024, Volume 1301

Yang, YH, Tan, JS, Wang, F, Sun, WM, Shi, HC, Cheng, Z, Xie, YC, Zhou, XH. Preconcentration and detection of SARS-CoV-2 in wastewater: A comprehensive review[J]. BIOSENSORS & BIOELECTRONICS, Published 2024, Volume 263

Zhou, Xiaohong,Zhu, Qian,Zheng, Anqi,Xue, Boyuan,Wang, Qihui,Chin, Lip Ket,Jiang, Jingkun,He, Miao. De novo design of covalent bonding peptides for target protein [J]. hLife, Published 2024, Volume 2, Issue 12, Pages 641-652

Liu, JC, Yang, H, Li, HX, Wang, JC, Zhou, XH. A colorimetric nanobiosensor with enhanced sensitivity for detection of lead (II) in real-water samples via an adenine-cytosine mismatched DNAzyme[J]. FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING, Published 2024, Volume 18, Issue 12

Xue, BY, Li, RX, Cheng, Z, Zhou, XH. High-Affinity Peptides for Target Protein Screened in Ultralarge Virtual Libraries[J]. ACS CENTRAL SCIENCE, Published 2024, Volume 10, Issue 11, Pages 2111-2118

Jin, YS, Xue, BY, Zhou, XH. Protein Biomarkers of DNA Damage in Yeast Cells for Genotoxicity Screening[J]. ENVIRONMENT & HEALTH, Published 2024, Volume 3, Issue 3, Pages 250-258

Liu, JC, Xing, YP, Lin, YS, Xie, YC, Zhou, XH. Effect of pretreatment approach on the ELISA-based detection of cyanotoxins in water: Analysis and application[J]. SCIENCE OF THE TOTAL ENVIRONMENT, Published 2023, Volume 871

Tan, Jisui, Chen, Yangyang, He, Jing, Occhipinti, Luigi G., Wang, Zhanhui, Zhou, Xiaohong. Two-dimensional material-enhanced surface plasmon resonance for antibiotic sensing[J]. Journal of Hazardous Materials[0304-3894], Published 2023, Volume 455

Wang, Xingwei, Zhou, Wenbo, Wang, Yidi, Gong, Likun, Liu, Xiaobin, Zhou, Xiaohong. MoO2/Mo heterostructures for hydrogen evolution reaction and ammonia sensing in self-powered mode[J]. Nano Energy, Published 2023, Volume 109

Li, Fangxu, Yang, Yihan, Tan, Jisui, Wang, Zhanhui, Zhou, Xiaohong. Group-targeting sulfonamides via an evanescent-wave biosensor based on rational designed coating antigen[J]. Science of the Total Environment, Published 2023, Volume 861

Dai, Zongren, Tan, Jisui, Zhou, Kaiming, Zhang, Lin, Zhou, Xiaohong, Tan, Yidong. Optical fiber SPR biosensor with frequency multiplexing compensated laser heterodyne feedback for ultrasensitive detection of fluoroquinolones[J]. Sensors and Actuators B: Chemical, Published 2023, Volume 393

Yang, Yihan, Wang, Fan, Xue, Boyuan, Zhou, Xiaohong. Field-deployable assay based on CRISPR-Cas13a coupled with RT-RPA in one tube for the detection of SARS-CoV-2 in wastewater[J]. Journal of Hazardous Materials, Published 2023, Volume 459

Yang, Qian, Zhu, Qian, Li, Fangxu, Gui, Ping, Zhang, Ruina, Liu, Chun, Zhou, Xiaohong. Genotoxicity detection technology and application based on recombinant luminescent yeast [J]. Huanjing Kexue Xuebao/Acta Scientiae Circumstantiae, Published 2023, Volume 43, Issue 8, Pages 464-472

Lin, Yongshu, Liu, Jinchuan, Tian, Yongjing, Huang, Tianyin, Hu, Guanjiu, Zhou, Xiaohong. Comparison of pre-treatment methods for microcystins detection based on the immunoassay [J]. Huanjing Kexue Xuebao/Acta Scientiae Circumstantiae, Published 2023, Volume 43, Issue 8, Pages 437-479

Tan, Jisui, Dai, Zongren, Zhou, Kaiming, Zhang, Lin, Zhou, Xiaohong, Tan, Yidong. Ultrasensitive Screening of Endocrine-Disrupting Chemicals Using a Surface Plasmon Resonance Biosensor with Polarization-Compensated Laser Heterodyne Feedback[J]. Analytical Chemistry, Published 2023, Volume 95, Issue 22, Pages 8687-8695

Tan, Jisui, Dai, Zongren, Zhou, Kaiming, Zhang, Lin, He, Miao, Tan, Yidong, Zhou, Xiaohong. An Ultrasensitive and Universal Surface Plasmonic Biosensor for Detection of Micropollutants in Aquatic Environments[J]. Environmental Science and Technology, Published 2023, Volume 57, Issue 22, Pages 8313-8322

Tan, Jisui, Zhou, Xiaohong. Detection of Retinoic Acid-Active Chemicals in Diverse Sample Matrices Via a Quantum Dots-Based Nuclear Receptor Fluorescence Probe-Mediated Biosensor[J]. Analytical Chemistry, Published 2023, Volume 95, Issue 20, Pages 8036-8044

Xue, Boyuan, Yang, Qian, Xia, Kaidong, Li, Zhihong, Chen, George Y., Zhang, Dayi, Zhou, Xiaohong. An AuNPs/Mesoporous NiO/Nickel Foam Nanocomposite as a Miniaturized Electrode for Heavy Metal Detection in Groundwater[J]. Engineering[2095-8099], Published 2023, Volume 27, Pages 199-208

Tan, JS, Li, FX, Liu, LH, Zhang, J, Gui, P, He, M, Zhou, XH. Effect-Targeted Mapping of Potential Estrogenic Agonists and Antagonists in Wastewater via a Conformation-Specific Reporter-Mediated Biosensor[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY, Published 2023, Volume 57, Issue 41, Pages 15617-15626

Xue, BY, Yang, Q, Jin, YS, Zhu, Q, Lan, JQ, Lin, YS, Tan, JS, Liu, LH, Zhang, T, Chirwa, EMN, Zhou, XH. Genotoxicity Assessment of Haloacetaldehyde Disinfection Byproducts via a Simplified Yeast-Based Toxicogenomics Assay[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY, Published 2023, Volume 57, Issue 44, Pages 16823-16833

H.H. Zhu, J. Zou, H. Zhang, Y.Z. Shi, S.B. Luo, N. Wang, H. Cai, L.X. Wan, B. Wang, X.D. Jiang*, J. Thompson, X.S. Luo, X.H. Zhou*, L.M. Xiao*, W. Huang, L Patrick, M. Gu*, L.C. Kwek*, and A. Q. Liu*. Space-efficient optical computing and an integrated chip of diffractive neural network. Nature Communications, 2022, 13, 1044.

Qian Zhu, Xiaohong Zhou*. A colorimetric sandwich-type bioassay for SARS-CoV-2 using a hACE2-based affinity peptide pair. Journal of Hazardous Materials, 2022, 425, 127923.

Jinchuan Liu, Yunpeng Xing, Boyuan Xue, Xiaohong Zhou*. Nanozyme enhanced paper-based biochip with a smartphone readout system for rapid detection of cyanotoxins in water. Biosensors and Bioelectronics, 2022, 205, 114099.

Lanhua Liu#, Xuejun Zhang#, Qian Zhu#, Kaiwei Li, Yun Lu, Xiaohong Zhou*, Tuan Guo*. Ultrasensitive detection of endocrine disruptors via superfine plasmonic spectral combs. Light: Science and Applications, 2021, 10, 181.

Qian Zhu, Lanhua Liu, Ruoyu Wang, Xiaohong Zhou*. A split aptamer (SPA)-based sandwich-type biosensor for facile and rapid detection of streptomycin. Journal of Hazardous Materials, 2021, 403, 123941.

Yihan Yang, Jinchuan Liu, Xiaohong Zhou*. A CRISPR-based and post-amplification coupled SARS-CoV-2 detection with a portable evanescent wave biosensor. Biosensors and Bioelectronics, 2021, 190, 113418.

Jinchuan Liu, Yihan Yang, Qian Zhu, Zhanhui Wang, Guanjiu Hu, Hanchang Shi, Xiaohong Zhou*. ELISA-Based Method for Variant-Independent Detection of Total Microcystins and Nodularins via a Multi-immunogen Approach. Environmental Science & Technology, 2021, 55, 19, 12984-12993.

Jinchuan Liu, Yunpeng Xing, Xiaohong Zhou*, George Y. Chen*, Hanchang Shi. Light-sheet skew rays enhanced U-shaped fiber-optic fluorescent immunosensor for microcystin-LR. Biosensors and Bioelectronics, 2021, 176, 112902.

Yangyang Chen#, Qian Zhu#, Xiaohong Zhou*, Ruoyu Wang, Zhenchuan Yang. Reusable, facile, and rapid aptasensor capable of online determination of trace mercury. Environmental International, 2021, 146, 106181.

Jinbiao Ma, Manman Du, Can Wang*, Xinwu Xie*, Hao Wang, Tie Li*, Shixing Chen, Lixia Zhang, Shun Mao, Xiaohong Zhou*, Min Wu. Rapid and sensitive detection of Mycobacterium tuberculosis by an enhanced nanobiosensor. ACS Sensors, 2021, 6(9), 3367-3376.

Xiaohong Zhou*, Abdul Ghaffar Memon, Weiming Sun, Fang Fang, Jinsong Guo*. Fluorescent probe for Ag+ detection using SYBR GREEN I and C-C mismatch. Biosensors, 2021, 11, 6.

Weiming Sun, Lanhua Liu, Abdul Ghaffar Memon, Xiaohong Zhou*, Hongwei Zhao*. Waveguide-based fluorescent immunosensor for the simultaneous detection of carbofuran and 3-hydroxy-carbofuran. Biosensors, 2020, 10, 191. Selected as Title Story.

Xiaohong Zhou*, Qian Zhu, Yihan Yang. Aptamer-integrated nucleic acid circuits for biosensing: Classification, challenges and perspectives. Biosensors and Bioelectronics, 2020, 165, 112422.

Xuewen Yi, Zhanqi Gao, Lanhua Liu, Qian Zhu, Guanjiu Hu*, Xiaohong Zhou*. Acute toxicity assessment of drinking water source with luminescent bacteria: Impact of environmental conditions and a case study in Luoma Lake, East China. Frontier Environmental Science and Engineering, 2020, 14(6), 109.

Yunpeng Xing, Qian Zhu, Xiaohong Zhou*, Peishi Qi. A dual-functional smartphone-based sensor for colorimetric and chemiluminescent detection: A case study for fluoride concentration mapping. Sensors and Actuators B: Chemical, 2020, 319, 128254.

Yunpeng Xing, Qian Zhu, Xiaohong Zhou*, Peishi Qi*. A gold nanoparticle-based colorimetric mercury(II) biosensor using a DNA probe with phosphorothioate RNA modification and exonuclease III-assisted signal amplification. Microchimica Acta, 2020, 187, 214.

Abdul Ghaffar Memon, Yunpeng Xing, Xiaohong Zhou*, Ruoyu Wang, Lanhua Liu, Siyu Zeng*, Miao He*, Mei Ma. Ultrasensitive colorimetric aptasensor for Hg2+ detection using Exo-III assisted target recycling amplification and unmodified AuNPs as indicators. Journal of Hazardous Materials, 2020, 384, 120948.

Ruoyu Wang#, Qiansen Zhang#, Yi Zhang, Hanchang Shi, Kim Truc Nguyen, Xiaohong Zhou*. Unconventional split aptamers cleaved at functionally essential sites preserve biorecognition capability. Analytical Chemistry, 2019, 91, 15811-15817. Selected as Supplementary Cover Article.

Kaidong Xia, Zhihong Li, Xiaohong Zhou*. Ultrasensitive detection of a variety of analytical targets based on a functionalized low-resistance AuNPs/beta-Ni(OH)2 nanosheets/Ni foam sensing platform. Advanced Functional Materials, 2019, 29, 1904922.

Ruoyu Wang, Xiyu Zhu, Yunpeng Xing, Abdul Ghaffar Memon, Hanchang Shi, Xiaohong Zhou*. Multitag-regulated cascade reaction: A generalizable ultrasensitive microRNA biosensing approach for cancer prognosis. ACS Applied Materials & Interface, 2019, 11, 36444-36448.

Yangyang Chen, Jinchuan Liu, James S. Wilkinson*, Xiaohong Zhou*. Optical biosensors based on refractometric sensing schemes: A review. Biosensors and Bioelectronics, 2019, 144, 111693.

Ruoyu Wang, Xueqi Wu, Xiyu Zhu, Hanchang Shi, Xiaohong Zhou*. A photoregulated split aptaswitch for small molecules with improved sensitivity. Chemical Communications, 2019, 55, 9555-9558.

Lanhua Liu, Xiaohong Zhou*, Yun Lu, Hanchang Shi, Mei Ma, Tong Yu. Triple functional small-molecule-protein conjugate mediated optical biosensor for quantification of estrogenic activities in water samples. Environment International, 2019, 132, 105091.

Qian Zhu, Lanhua Liu, Xiaohong Zhou*, Mei Ma. In silico study of molecular mechanisms of action: Estrogenic disruptors among phthalate esters. Environmental Pollution, 2019, 255, 113293.

Xiyu Zhu, Ruoyu Wang, Kaidong Xia, Xiaohong Zhou*, Hanchang Shi. Nucleic acid functionalized fiber optic probes for sensing in evanescent wave: optimization and application. RSC Advances, 2019, 9, 2316-2324.

Abdul Ghaffar Memon, Xiaohong Zhou*, Yunpeng Xing, Ruoyu Wang, Lanhua Liu, Mohsin Khan, Miao He*. Label-free colorimetric nanosensor with improved sensitivity for Pb2+ in water by using a truncated 8-17 DNAzyme. Frontiers of Environmental Science & Engineering, 2019, 13, 12.

Lanhua Liu#, Didi Shan#, Xiaohong Zhou, Hanchang Shi, Baodong Song, Floris Falke, Arne Leinse, R.G. Heideman. TriplexTM waveguide-based fluorescence biosensor for multichannel environmental contaminants detection. Biosensors and Bioelectronics, 2018, 106, 117-121.

Jinchuan Liu, Xiaohong Zhou*, Hanchang Shi. An optical biosensor-based quantification of microcystin synthetase A gene: Early warning of toxic cyanobacterial blooming. Analytical Chemistry, 2018, 90, 2362-2368.

Lanhua Liu, Xiaohong Zhou*, Ruiman Ma, Miao He, Hanchang Shi, Qian Yi. High-throughput biomolecular interaction analysis probing by an array fluorescent biosensor platform, Sensors and Actuators B: Chemical, 2018, 259,888-893.

Qian Zhu, Lanhua Liu, Yunpeng Xing, Xiaohong Zhou*. Duplex functional G-quadruplex/NMM fluorescent probe for label-free detection of lead(II) and mercury(II) ions, Journal of Hazardous Materials, 2018, 355, 50-55.

Ruoyu Wang, Xiaohong Zhou*, Bo Liedberg, Xiyu Zhu, Abdul Ghaffar Memon, Hanchang Shi. Screening criteria for qualified antibiotic targets in unmodified gold nanoparticles-based aptasensing. ACS Applied Materials & Interfaces, 2017, 9, 35492–35497.

Xiyu Zhu, Ruoyu Wang, Xiaohong Zhou*, Hanchang Shi. Free-energy-driven lock/open assembly-based optical DNA sensor for cancer-related microRNA detection with a shortened time-to-result, ACS Applied Materials & Interface, 2017, 9, 25789-25795.

Lanhua Liu, Xiaohong Zhou*, Yun Lu*, Didi Shan, Bi Xu, Miao He, Hanchang Shi, Yi Qian. Facile screening of potential xenoestrogens by an estrogen receptor-based reusable optical biosensor. Biosensors and Bioelectronics, 2017, 97, 16-20.

Lanhua Liu, Xiaohong Zhou*, James S. Wilkinson, Ping Hua*, Baodong Song, Hanchang Shi. Integrated optical waveguide-based fluorescent immunosensor for fast and sensitive detection of microcystin-LR in lakes: optimization and analysis. Scientific Reports, 2017, 7: 3655.

Ruoyu Wang, Xiaohong Zhou*, Xiyu Zhu, Chao Yang, Lanhua Liu, Hanchang Shi. Isoelectric bovine serum albumin: Robust blocking agent for enhanced performance in optical-fiber based DNA sensing. ACS Sensors. 2017, 2(2): 257–262.

Abdul Ghaffar Memon, Xiaohong Zhou*, Jinchuan Liu, Ruoyu Wang, Lanhua Liu, Bofan Yu, Miao He*, Hanchang Shi. Utilization of unmodified gold nanoparticles for label-free detection of mercury (II): Insight into rational design of mercury-specific oligonucleotides. Journal of Hazardous Materials, 2017, 321, 417-423.

Lanhua Liu, Xiaohong Zhou*, Mifang Lu, Mingkai Zhang, Chao Yang, Ruiman Ma, Abdul Ghaffar Memon, Hanchang Shi, Yi Qian. An array fluorescent biosensor based on planar waveguide for multi-analyte determination in water samples. Sensors and Actuators B: Chemical, 2017, 240, 107-113.